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. 2022 Aug 29;27(17):5545. doi: 10.3390/molecules27175545

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Resveratrol treatment improves the neonatal rat cardiomyocytes mitophagy induced by H/R, while this effect was abrogated by EX527. (A) Confocal fluorescent microscopic images of cardiomyocytes revealed the staining of mitochondrial autolysosomes. (B) Western blot was performed with cardiomyocytes lysate for expression of LC3, PINK1 and Parkin. (C) The interaction of Parkin with P62 was investigated by co-immunoprecipitation (Co-IP). Figures are representative images of three different samples. Results are expressed as mean ± S.D. * p < 0.05 vs. control group; ^# p < 0.05, ^## p < 0.01 vs. H/R group; ^∆ p < 0.05, ^∆∆ p < 0.01, ^∆∆∆ p < 0.001 vs. Res-20 group.