Figure 1.

Growth factors elicits cluster formation in human olfactory neuronal precursors. ONPs were incubated during 6 h with 20 ng/mL of either fibroblastic growth factor (FGF), epidermal growth factor (EFG), brain-derived neurotrophic factor (BDNF) or the vehicle (VEH). Then, cells were fixed and stained with anti-doublecortin and anti-nestin antibodies, followed by secondary antibodies coupled to FITC (green) and RITC (red), respectively. Representative images are shown in Panel A. Another group of cells was stained with DAPI and an anti-Ki67 antibody, followed by a secondary antibody that recognizes the anti-Ki67 antibody (Panel B). Scale bar = 50 µm.