Figure 1.

Dose‒response curve of chemotherapy in cells differentially expressing the P2X7 receptor and its isoforms. Dose–response curves of vincristine (n=6), doxorubicin (n=3) and cyclophosphamide (n=3) in neuroblastoma cells. (A) Viability assays and (B) cell death assays performed in ACN cells containing scrambled shRNA. (C) Drug-response curves of the chemotherapy drugs vincristine, doxorubicin and cyclophosphamide in HEK 293 cells obtained by PI staining quantification by flow cytometry. (D) Cell viability measurements of ACN cells expressing (scrambled), silenced for both A and isoforms (shRNA1) and silenced for B isoform (shRNA2) after treatment with vincristine or doxorubicin. Data normalized by the respective cell type untreated control (n>=3). *p<0.05; **p<0.01; ***p<0.001.