Table 3.
Identity, sources, and the working dilution of antibodies used in immunohistochemical studies.
| Target | Primary Antibody Supplier | Origin (Catalog No) | Dilution | Incubation | Antigen Retrieval | Secondary Antibody-Incubation Time |
|---|---|---|---|---|---|---|
| COX-2 | Abcam | Rabbit anti-mouse monoclonal [EPR12012] to COX2 | 1:100 | Overnight | Boiling in citrate buffer (pH 6.0), 20 min Goat |
Goat anti-Mouse IgG (H+L) Secondary Antibody Catalog # 31569 Dilution; 1:100 One hour at room temperature |
| IL- 1β | Bio-Rad | Rabbit anti-Mouse Interleukin-1 beta Clone: AAM13G Polyclonal |
1:200 | Overnight | Boiling in citrate buffer (pH 6.0), 20 min | |
| IL-6 | Abcam | Mouse monoclonal Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) |
1:100 | 45-min incubation at room temperature | Boiling in citrate buffer (pH 6.0), 20 min | |
| TNF-α | (Novus Biologicals, Littleton, CO, USA), | TNF-alpha Antibody [NBP1-19532] | 1:300 | Overnight | Boiling in citrate buffer (pH 6.0), 20 min | |
| PCNA | Anti PCNA Santa Cruz Biotechnology, Inc. | (PC10 sc-56 mouse anti-rat IgG2a monoclonal antibody | 1:500 | 2 h at room temperature | Boiling in citrate buffer (pH 6.0), 20 min |