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. 2022 Aug 25;27(17):5430. doi: 10.3390/molecules27175430

Figure 5.

Figure 5

Shear pulses and resveratrol sensitize SW480 cells to TRAIL individually but have minimal synergistic effects. (A) Phase contrast images of SW480 cells 24 h after FSS treatment with 50 ng/mL TRAIL and 50 µM resveratrol. Samples exposed to shear pulses and treated with TRAIL + RSV displayed an increased number of apoptotic cells, as visualized by a rounded and shrunken morphology. Scale bar = 100 µm. (B,C) The viabilities of SW480 and SW620 cells measured via AnnexinV/PI staining following FSS treatments with or without 50 ng/mL TRAIL and 50 µM resveratrol. N = 3, * p < 0.05, ** p < 0.01, *** p < 0.001 (two-way ANOVA with multiple comparisons). Error bars represent mean ± SD. (D) Ratiometric fluorescence of Fluo-4/FuraR as a metric of intracellular calcium concentration in real time. Cells were sheared with one pulse at t = 0 then immediately analyzed via flow cytometry. (E,F) Insignificant changes in median fluorescence intensity of ratiometric calcium fluorescence for SW480 and SW620 cells, respectively. Median fluorescence was calculated over the course of the first 200 sec. N = 3 (two-way ANOVA with multiple comparisons). Error bars represent mean ± SD.