TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| pMU720 | Miniplasmid derived from pMU707; Gal IncB | 8 |
| pMU604 | Miniplasmid derived from pMU407.1; Gal IncL/M | 11 |
| pMU605 | Miniplasmid derived from R64-11; Gal IncI1 | 11 |
| pMU2200 | Miniplasmid derived from pIE545; Gal IncZ | 31 |
| pMU2209 | Miniplasmid derived from R387; Gal IncK | 31 |
| pMU1598 | pAM34 carrying nt 1–2170 of pMU720 with a T643C copy up mutation; Ap Cm lacIq IncB | 30 |
| pMU1599 | pAM34 carrying nt 1–2170 of pMU720; Ap Cm lacIq IncB | 30 |
| pMU1583 | pSU39 carrying nt 1–1759 of pMU720 with T642C and T640C mutations; Km IncB; RepA plasmid | This study |
| pMU1584 | pSU39 carrying nt 1–1843 of pMU720 with T642C and T640C mutations; Km IncB; RepA plasmid | This study |
| pMU1585 | pSU39 carrying nt 1–1916 of pMU720 with T642C and T640C mutations; Km IncB; RepA plasmid | This study |
| pMU1586 | pAM34 carrying nt 1916–2170 of pMU720; Ap Cm lacIq; ori plasmid | This study |
| pMU1587 | pAM34 carrying nt 1840–2170 of pMU720; Ap Cm lacIq; ori plasmid | This study |
| pMU1588 | pAM34 carrying nt 1759–2170 of pMU720; Ap Cm lacIq; ori plasmid | This study |
| pMU1589 | pAM34 carrying nt 1825–2160 of pMU604; Ap Cm lacIq; ori plasmid | This study |
| pAM34 | pMB1 derivative in which the preprimer RNA is expressed from lacZpo; lacIq Ap Sp | 13 |
| pSU39 | p15A replicon; Km | 7 |
a
Abbreviations: Ap, ampicillin resistance; Cm, chloramphenicol resistance; Km, kanamycin resistance; Sp, spectinomycin resistance; nt, nucleotides. Mutations introduced into RepA and ori plasmids are described in Results.