TABLE 6.
Changes in the stability of the tnaC secondary structure do not correlate with expression of the tna operon
| Mutational change | tna operon expression | Change in tnaC secondary structure stabilitya (kcal/mol) |
|---|---|---|
| UUC12 (W→F) | Noninducible | +2.9 |
| UGA12 (W→stop) | Noninducible | +4.2 |
| UAG12 (W→stop) | Noninducible | +3.6 |
| AUG12 (W→M) | Noninducible | +3.6 |
| UGC13 (F→C) | Noninducible | +3.1 |
| UUU15 (I→F) | Noninducible | +0.6 |
| AAU15 (I→N) | Noninducible | +2.8 |
| Δ(CGC23CCU24) | Constitutive | +1.7 |
| AGC23 (R→S) | Constitutive | −0.8 |
| CGG23 (R→R) | Constitutive | +0.8 |
| AGA23 (R→R) | Constitutive | −1.3 |
| AGG23 (R→R) | Constitutive | −0.8 |
| UGG13 (F→W) | Constitutive | −6 |
| AUA15 (I→I) | Constitutive | +2.6 |
| AUC15 (I→I) | Constitutive | −2.1 |
| AUG15 (I→M) | Constitutive | +1.7 |
| CAC16 (D→H) | Constitutive | +5.5 |
| UUC16 (D→F) | Constitutive | +5.7 |
| CGA23 (R→R) | Regulated | −1.3 |
| CGU23 (R→R) | Regulated | −1.6 |
| CCA24 (P→P) | Regulated | +0.1 |
| CCG24 (P→P) | Regulated | +0.1 |
| UUU13 (F→F) | Regulated | 0 |
| GAU16 (D→D) | Regulated | +1.9 |
| AAC14 (N→N) | Regulated | 0 |
| ACU24 (P→T) | Regulated | +0.3 |
| UCU24 (P→S) | Regulated | +0.3 |