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Principle of the iso-FRET competition assay. The modified strands (37Q, F22) are shown in color. In the first scenario (top) (i), the competitor (grey) forms a quadruplex and traps PhenDC3 (orange oval), allowing 37Q (red) to quench F22 (green) by forming a duplex. In the second scenario (bottom) (ii), the competitor does not form a quadruplex, PhenDC3 remains bound to 37Q, which cannot hybridize to F22 which emits a strong fluorescence signal.
