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Time-dependent fluorescence of the F22 + 37Q system at different potassium concentrations. Concentration of F22 and F22m were 20 nM, 37Q and 37Qm were 200 nM. 37Q and 37Qm were kept in corresponding buffer for 5 min before adding F22 or F22m, respectively. Hybridizations were processed at RT. Panel A and B related to the same normalized fluorescence scale.
