Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 26;50(16):9568–9579. doi: 10.1093/nar/gkac682

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — RNAI structure, sequence comparison between common colE1 origins and RNAI libraries. (A) The predicted structure for RNAI, generated by the ViennaRNA suite (38), consists of three stem loops named (5′→3′) Hairpin III, II, and I. Nucleotides are coloured based on their position in the structure, whether in stem (green), loop (blue), stem bulges and mismatches (yellow), and unpaired (red). (B) Natural plasmids from different compatibility groups show similarities in sequence (shown as ‘.’ in comparison to colE1 origin in pET29) in both stem and loops (highlighted in cyan). (C) Libraries were designed to introduce sequence variation (red) around loop regions. Sequence degeneracies are shown as per IUPAC (N = A, C, G or T; K = C, T).