Figure 1.

Sample specificity of Ribo-seq produced rRNA fragments. (A) PCA with position-specific rRNA abundance measures (regularized log of normalized count) shows that Ribo-seq produced rRNA fragments are consistently different across fetal tissues. (B) Schematic representation of different ribosome populations that are subjected to Ribo-seq in (2). (C) PCA with rRNA fragments from different ribosome populations shows consistent and clear separation of samples. (D) Comparison of position-specific rRNA abundances between selected populations shows the consistency across replicates (n = 2, separate lines in rows 1, 2, 4 and 5) and reveals that hotspots (highlighted) for rRNA fragment heterogeneity change across comparisons. Note that rows 3 and 6 indicate the differences between populations in rows 1, 2 and 4, 5, respectively.