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. 2022 Jun 10;50(16):e95. doi: 10.1093/nar/gkac484

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — eS25/RPS25 is heterogeneous between fetal and adult ribosomes in mice, with a potential consequence on selective mRNA translation. (A) Enrichment score 1 (RPSEA) heatmap for different RPs (rows) in dripARF ribosome heterogeneity predictions in selected comparisons (columns). Predictions are based on Ribo-seq data (20), generated with the adult and fetal stage of 6 organs in mice; heart, retina, liver, kidney, lung and brain. (B) Closer look at rRNA fragment abundance change at rRNA positions in the eS25/RPS25 vicinity shows consistency across fetal-vs-adult comparisons. In this figure, the layout is the same as Figure 2C. (C) Translation efficiency changes in adult-vs-fetal comparisons, and mRNA association with eS25-enriched ribosomes is significantly associated with mRNA CDS length. In the figure, CDS length (y-axis) distribution of genes are given separately for each comparison, where genes are separated on the x-axis, into different TE groups for the first dataset and into association groups with specific ribosome populations for the second dataset.