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Piezo1 KO reduces proliferation of E10.5 NSCs in vitro. Time-lapse fluorescence and phase contrast imaging of WT and Piezo1 KO NSCs over 60 h. Top: Nuclei stained with siR-DNA (red). Bottom: Phase contrast imaging overlaid with nuclear stain. Images collected every 2 h. Video frame rate is 3 frames/s. Representative images from four images per three wells for each of n = 4 embryos for WT and n = 3 embryos for Piezo1 KO. Related to Fig. 3, D and E.
