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Purification of AlgSHis6: SDS-PAGE of fractions obtained at various stages of purification, stained with Coomassie blue. Lanes 1 and 6, size markers (in kilodaltons); lane 2, soluble fraction; lane 3, column flowthrough; lane 4, wash I; lane 5, wash II; lane 7, imidazole eluate. Proteins were loaded at 5.8 μg for lanes 2, 3, and 4 and 1 μg for lane 7. For lane 5, the same volume of the protein solution as in lane 4 was loaded.
