TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Sphingomonas sp. | ||
| A1 | Wild type | 23 |
| AL-L | Alginate uptake-deficient mutant | This study |
| A1dS | algS::Kmr | This study |
| E. coli | ||
| DH5α | General cloning host strain | TOYOBO |
| HB101 | General cloning host strain | CLONTECH |
| BL21(DE3)pLysS | General protein expression strain | Novagen |
| Plasmids | ||
| pKS13 | Tetrcos Mob+ RK2 replicon | 14 |
| pKTY320 | Ampr Cmr Mob+ p15A replicon | 14 |
| pRK2013 | Kmr Tra+ ColE1 replicon | CLONTECH |
| pET3a | E. coli expression vector, Ampr | Novagen |
| pET14b | E. coli expression vector, Ampr | Novagen |
| pBluescriptII SK+ | E. coli cloning vector, Ampr | Stratagene |
| pBE11 | 23-kb Sau3AI fragment of strain A1 genomic DNA in pKS13 | This study |
| pALC1 | 2.8-kb PstI fragment in pKS13 | This study |
| pALC2 | 6-kb EcoRI fragment in pKS13 | This study |
| pAS3 | algS in pET3a | This study |
| pAS4 | algS in pET14b | This study |