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recA transcription in the recX mutant SVX1. Following induction with MMS, the transcription of recA was analyzed by RT-PCR using primers recA1 and recA2 (Fig. 1). Lanes: DNA, control PCR using genomic DNA as a template; 0′, without induction; 20′, 40′, and 60′, 20, 40, and 60 min after induction, respectively; lane M, size standard (1-kb ladder [Boehringer]).
