TABLE 1.

Plasmids used in this work

Plasmid	Description	Reference or source
pUC18	lacZ bla	31
pGM11	aphII; temperature-sensitive Streptomyces vector	37
pJF293.2	bla; PtipA	J. Altenbuchner, Stuttgart, Germany
pSVX1	recX replacement plasmid; pGM11 derivative carrying the 1,550-bp PstI-NcoI fragment; tsr insertion within the BclI site	This study
pSVQ1	Recombination test plasmid; pGM11 derivative carrying a 1,316-bp recQ PCR fragment, disrupted by the tsr gene	This study
pSVX2	pGM11 derivative carrying the 1,685-bp SalI fragment encoding RecX and the C-terminal half of RecA	This study
pSVAX2	recX complementation plasmid; pGM11 derivative carrying a 2,346-bp PCR fragment containing the complete recA-recX operon	This study
pIJ4123	Streptomyces His tag expression vector; tsr kan PtipA redD	30
pSVX-his	pIJ4123 derivative carrying a PCR fragment containing the recX gene	This study
p2001/41	Bifunctional SCP2 derivative, p15a E. coli ori, tsr cat	Unpublished data
pEXrecA	recA expression plasmid; p2001/41 derivative; PtipA recA tsr aacC1	This study
pEXR169-H	recA expression plasmid; p2001/41 derivative; PtipA recA(R169-H)	This study