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. 2022 Sep 8;12:15058. doi: 10.1038/s41598-022-18324-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Propranolol modulates epidermal growth factor-induced actin cytoskeleton reorganization and stress fiber formation in osteosarcoma cells. Representative images of MG-63 cells stained with AlexaFluor555-conjugated phalloidin (red) after exposure to saline vehicle (unstimulated control), epidermal growth factor (EGF) (control), and PPN 10 or 50 µM, in addition to EGF stimulation. Nuclei were counterstained with DAPI (blue). Actin fluorescent staining in MG-63 cells grown was assessed by confocal laser scanning microscopy. Insets contain enlarged representative cells indicated by dashed squares. (×630 magnification. Scale bar = 20 µm).