FIG. 2.

Mapping of mutations shown in Fig. 1 by recombination. Mutants with chromosomal fimH alleles shown on the right side of the figure were incubated with the plasmids shown at the left. Recombinants capable of hemagglutination were recovered following enrichment as described in the text. The fimH gene is denoted by the diagonally striped rectangle. The dotted line indicates the region deleted. Specific deletions (codons deleted) from the 3′ end of FimH are listed in Table 1. Deletions were created by removing either the restriction fragments indicated or by using Bal31 exonuclease digestions starting from the XhoI site in pORN118 as described in the text.