FIG. 3.

Microtiter agglutination reactions of strain ORN157 bearing the fimH205 allele compared to the parental strain (ORN155) and strain ORN133, a fimH insertion mutant (fimH′-kan). (A) Bacterial agglutination of mutants grown at the temperatures indicated, 31 or 42°C, concentrated twofold from overnight cultures, and incubated with FimH antiserum (1:4 dilution) as described in the text. (B) Microtiter guinea pig erythrocyte agglutination by the same strains obtained under the same growth condition. Incubation conditions are described in the text.