FIG. 5.

Comparison of the binding ability of mutants having the fimH lesions indicated relative to a fimH insertion mutant (fimH′-kan strain ORN133, denoted as FimH⁻ in the legend box) and compared to the parental (ORN155) strain for their ability to bind guinea pig erythrocytes (RBCs) and resident BALB/c peritoneal macrophages. All mutant strains were grown at 37°C except for the fimH205 mutant, which was grown at the temperatures indicated. Parental binding values (ratio of the parental strain to the fimH insertion mutant; far right-hand bars were not significantly affected by the growth temperatures employed (31, 37, or 42°C), and for economy, a single average value is shown. Vertical bars represent the standard error of the mean. The dashed line indicates a 1:1 ratio between the test strain and the fimH insertion mutant. At least two experiments were averaged.