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. 2022 Sep 8;5:931. doi: 10.1038/s42003-022-03898-5

Fig. 3. DHA in combination with colistin effectively kills the tested bacteria.

Fig. 3

a, b Fluorescence labeling analysis for E. coli W3110 (pUC19-mcr-1) (a) and S. typhimurium HYM2 (b) were treated with DHA (32 µg/mL), colistin (0.5/1 µg/mL) the combination or control (bacteria without any treatment), and live bacteria were dyed green and dead bacteria were dyed red (scale bar = 50 μm). c, d SEM analysis (scale bar = 5 μm) for E. coli W3110 (pUC19-mcr-1) (c) and S. typhimurium HYM2 (d) was also performed with the above treatment (5.00 kV, bar = 5 μM, 10 μM). Only in the control group and 1 μg/mL colistin treatment group observed the filamentous flagella at both ends of the S. typhimurium (yellow dashed circles). The experiments were repeated three times independently, and representative data are displayed.