Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 8;13:5167. doi: 10.1038/s41467-022-32861-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Schematic and quantification of fork degradation assays in control (sgCTRL) and REV3L-depleted HeLa cells, transduced with the indicated shRNAs and treated with 4 mM HU for 4 h. b Schematic and quantification of ssDNA-positive cells after CldU labeling for 16 h, followed by mock treatment (open circles) or treatment with 4 mM HU for 2 h (closed circles). Bars represent the mean ± SD of three independent experiments. c Quantification of survival assays in control and REV1-depleted HeLa cells, untreated or treated with 0.5 μΜ MMC or 1 μΜ CIS. Each dot represents one of three independent experiments. d Schematic and quantification of EdU incorporation intensity in control and REV1-depleted HeLa cells, untreated or treated with 5 μΜ MMC for 24 h, followed by EdU labeling for 2 h. White bars represent the median of three independent experiments. e Schematic and quantification of fork progression assays in control and REV1-depleted HeLa cells, untreated or treated with 4 mM HU for 4 h. f Schematic and quantification of fork degradation assays in control and REV1-depleted HeLa cells, treated with 4 mM HU ± 50 μΜ mirin for 4 h. Open circles, no treatment; closed circles, treatment. g Schematic and quantification of fork restart assays in control and REV1-depleted HeLa cells, treated with 4 mM HU for 1 h as indicated. Bars represent the mean ± SD of three independent experiments. h Schematic and quantification of fork degradation assays in control and REV1-depleted HeLa cells transduced with control shRNA (shSCR, open circles) or MAD2L2 shRNA (closed circles). Experimental conditions were similar as in (a). Pink bars in fiber plots represent the mean. Statistical analysis in (a, d, e, f, h) was performed according to two-tailed Mann-Whitney test. A representative fiber experiment from two (h) or three (a, d, e, f) independent biological replicates is shown. Additional replicates and combined fiber plots are provided in the Supplementary Information. Statistical analysis in (b, c, g) was performed according to one-way ANOVA with Dunnett’s multiple comparisons test.