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. 2022 Sep 9;558:111775. doi: 10.1016/j.mce.2022.111775

Fig. 2.

Fig. 2

Overexpression of ERα, ERβ and GPER1 does not significantly module ACE2 and TMPRSS2 in BEAS-2B cells. However, estrogen receptors overexpression revealed the participation of GPER1 in the modulation of viral load in BEAS-2B cells. A) Histogram of gene expression by RT-qPCR of ESR1 (A1), ESR2 (A2) and GPER1 (A3) between plasmid control cells group (plasmid containing Luciferase) and nucleofected cells group with plasmids containing ESR1, ESR2 and GPER1 genes, respectively, after 48 h of incubation. Data were normalized to the RPL35 gene. B, C) Histogram of gene expression by RT-qPCR of ACE2 (B) and TMPRSS2 (C) in cells that overexpress ERα, ERβ, GPER1 or Luciferase (control plasmid) after 24 h of infection with SARS-CoV-2 or not (mock). D) Histogram of gene expression by RT-qPCR (N gene) of cell extract from groups overexpressing Luciferase (plasmid control), ERα, ERβ or GPER1, after 24 h of infection with SARS-CoV2 (MOI 0.2). N gene expression data were normalized in relation to the RNAse P gene. Values correspond to mean ± SEM. Significant difference was considered, with *p ≤ 0.05 - one-way ANOVA, with Tukey's post-test or Student's unpaired t-test. N = 3 (A1, A2, A3, D), N = 4 (B), N = 6 (C).