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. 2022 Sep 6;34(9):1248–1263.e6. doi: 10.1016/j.cmet.2022.07.014

Figure 1.

Figure 1

Generation of iNs from patient-derived fibroblasts

(A and B) Schematic: generation of iNs from fibroblasts (A) and PSA-NCAM-based FACS purification (B).

(C) Phase-contrast images of fibroblasts at day 0 (overconfluent) and at different stages of conversion. Scale bars, 10 μm.

(D and E) Immunostainings of βIII-tub and NeuN (D) and quantification of βIII-tub and NeuN-positive cells (E) per DAPI (control, n = 7; AD, n = 5), showing median and quartiles. Significance: unpaired t test, ∗p < 0.05 (E).

(F) Representative voltage responses of iNs elicited by current step stimulation. Arrows indicate distinctive features associated with specific intrinsic membrane currents. Blue, inward rectification; red, Ca spike; olive, depolarizing voltage sag; green, rebound depolarization.