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. 2022 Aug 26;13:959938. doi: 10.3389/fphar.2022.959938

FIGURE 4.

FIGURE 4

Myricetin-induced pyroptosis depends on Caspase-3. (A) Expression of cleaved caspase-3 and GSDME after myricetin treatment for 12 and 24 h and its statistical analysis. (B) Microscopic imaging of NCI-H446 and A549 cells treated with myricetin and a caspase-3 inhibitor (Z-DEVD-FMK, 20 μM) or myricetin alone. (C) NCI-H446 and A549 cells treated with caspase-3 inhibitor alone, myricetin alone, myricetin and caspase-3 inhibitor or mock. Expression of cleaved caspase-3 and GSDME and its statistical analysis. (D) Percentage of NCI-H446 and A549 cells with pyrotosis (stained with Annexin V+/PI+) and its statistical analysis. (E) Detection of LDH release levels. Results were presented as means ± standard deviation (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, when compared with the control).