FIG. 1.

Polymerization of M. tuberculosis FtsZ. M. tuberculosis FtsZ (10 μM) was assayed under the same conditions as those used for E. coli FtsZ (22). The protein was incubated in 50 mM MES-NaOH (pH 6.5)–10 mM MgCl₂–25 mM KCl in a fluorometer cuvette at 30°C. The 90° angle light scattering was monitored to obtain a baseline. After 6 min, 1 mM GTP was added (●). The dynamic nature of the assay is shown in the other curve (○), in which the addition of 1 mM GTP was followed by 20 mM EDTA, 25 mM MgCl₂, 1 mM GTP, and finally 20 mM EDTA.