FIG. 6.

Effect of pH on FtsZ polymerization. FtsZ (13 μM) was polymerized at three pH values in a constant-ionic-strength buffer (50 mM HEPES, 50 mM MES, 100 mM ethanolamine) containing 100 mM KCl and 5 mM MgCl₂. Polymerization was initiated by the addition of 0.04 mM GTP (arrow).