FIG. 8.

Time course for GTP hydrolysis of M. tuberculosis FtsZ. At various times, 25-μl aliquots were withdrawn and assayed for release of ³²P. The kinetics of GTP hydrolysis with 100 mM KCl and 5 mM MgCl₂ were determined at a concentration slightly above the minimum concentration required for polymerization (5 μM) and at a concentration that gave both polymerization and depolymerization within 60 min (13 μM; Fig. 7). Inhibition by SRI 7614 (10 mM, six times the IC₅₀ value) was determined with 13 μM FtsZ. (Inset) Expansion of the time course for 5 μM FtsZ.