FIGURE 3.

hCB1R-dependent inhibition of forskolin-stimulated cAMP accumulation. Compound activity was quantified for inhibition of 10 µM forskolin-stimulated cAMP accumulation in CHO cells stably expressing hCB1R and treated with 0.1 nM—10 µM (A) cannabinoids; (B) cannabinoids + 100 nM ∆⁹-THC; (C) phytomolecules; and (D) phytomolecules + 100 nM ∆⁹-THC for 90 min according to the standard operating procedures of the HitHunter assay. Data were fit to a variable slope (four parameters) non-linear regression in GraphPad (v. 9). n ≥ 6 independent experiments were performed in triplicate. Data are expressed as mean ± SEM. EC₅₀ and E_max are reported in Table 1.