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. 2022 Aug 26;13:956030. doi: 10.3389/fphar.2022.956030

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Copyright © 2022 Zagzoog, Cabecinha, Abramovici and Laprairie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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hCB1R-dependent inhibition of forskolin-stimulated cAMP accumulation. Compound activity was quantified for inhibition of 10 μM forskolin-stimulated cAMP accumulation in CHO cells stably expressing hCB1R and treated with compounds as indicated for 90 min according to the standard operating procedures of the HitHunter assay. Data were analyzed in GraphPad (v. 9). n = 6 independent experiments were performed in triplicate. Data are expressed as mean ± SEM. **p < 0.01, ****p < 0.0001 as determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons.