Table 1.

Primer pairs used for polymerase chain reaction.

Target	Primer names	Sequences	Product length	GC%	Tm
RLEP	RLEP-F RLEP-R	5`-TGCGCTAGAAGGTTGCCGTAT-3` 5`-ATTTCTGCCGCTGGTATCGGT-3`	148	52.38 52.38	62.17 62.19
folP1	folP1-F1 folP1R1	5` - CTTGATCCTGACGATGCTGT - 3` 5` - CCACCAGACACATCGTTGAC - 3`	254	50.00 50.00	57.69 58.85
folP1	folP1-F2 folP1-R2	5` - GATCCTGACGATGCTGTCCAG - 3` 5` - ACATCGTTGACGATCCGTG - 3`	242	57.14 52.63	60.54 57.97
rpoB	rpoB-F1 rpoB-R1	5` - ACGCTGATCAATTATCCGTCC - 3` 5` - GTATTCGATCTCGTCGCTGA - 3`	345	47.62 50.00	58.24 57.33
rpoB	rpoB-F2 rpoB-R2	5` - CTGATCAATATCCGTCCGGT - 3` 5` - CGACAATGAACCGATCAGAC - 3`	255	50.00 50.00	56.89 56.65
gyrA	gyrA-F1 gyrA-R1	5` - ATGACTGATATCACGCTGCCA - 3` 5` - ATAACGCATCGCTGCCGGTGG - 3`	390	47.62 61.90	59.59 65.97
gyrA	gyrA-F2 gyrA-R2	5`- GATGGTCTCAAACCGGTACATC - 3` 5` - ACCCGGCGAATTGAAATTG - 3`	225	50.00 47.37	58.80 56.89

RLEP, Repetitive element; folP1, dapsone resistance-associated target; rpoB, rifampicin resistance-associated target; gyrA, quinolone resistance-associated target.