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PP50 made the strongest property on inguinal LNs targeting and uptake by DCs in vivo. (A) Female BALB/c mice treated with Blank (control), DiR-loaded PP0, PP25, PP50, PP75 or PP100. Female BALB/c mice were observed by fluorescence images. (B) The heart, liver, spleen, lung, kidney and inguinal LNs were observed by fluorescence images. (C) Inguinal LNs were observed by ROI analysis at 24 h post-administration. (D) Percentages of DCs in inguinal LNs that were positive for DiR: CD11c+DCs.
