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. 2021 Aug 30;46(5):636–645. doi: 10.1016/j.jgr.2021.08.004

Fig. 3.

Fig. 3

ZFP91 knockdown reduced gemcitabine resistance of PAAD cells. A.ZFP91 mRNA (up) and protein (bottom) expression in PANC-1 and PANC-1/GR cells. B. ZFP91 protein expression 48 h after lentivirus-mediated ZFP91 knockdown. C. Gemcitabine IC50 of PANC-1/GR cells with or without ZFP91 knockdown. D-E. PANC-1/GR cells were subjected to lentiviral infection for ZFP91 knockdown. 24 h later, the cells were further treated with 100 nM gemcitabine for 48 h. Then, cells were then harvested, stained by Annexin/PI and analyzed by flow cytometry. Representative flow cytometric images (D) were shown and the percentage of apoptotic cells (means ± SD) (E) were calculated. F-G. PANC-1/GR cells after the treatment as indicated in panel D were subjected to IF staining of γ-H2AX foci formation. Representative images (F) and quantitation (G) of γ-H2AX foci formation were shown. H. Western blot analysis of Bax, Bcl-2, cleaved caspase-3 and γ-H2AX expression in PANC-1/GR cells after the treatment as indicated in Fig. 3D. Scale bar: 20 μm.