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. 2022 Aug 26;16:982596. doi: 10.3389/fnins.2022.982596

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Copyright © 2022 Shi, Wang, Li, Zhang, Qu, Dai and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VN GABAergic neurons in the Vgat-ires-cre mice with a modified AAV-based, anterograde tracing vector. (A) Schematic diagram of anterograde tracing of VN GABAergic neurons using AAV vector, and the overall outputs distribution of VN GABAergic neurons (sagittal). (B) After injection into the VN, schematic representation of positive GABAergic neurons (red); (C,D) Typical figures showing the injection site of the recombinant anterograde vector for VN GABAergic neurons of VGAT-IRES-Cre mice. Scale Bar: 100 μm.