Figure 3.

Enhanced numbers of resident memory T cells were observed in the lungs of mice immunized with ID93 combined with GLA liposomes and QS-21. Mice were immunized with the ID93 therapeutic vaccines 4 weeks after the start of drug treatment, three times, 3 weeks apart. Four weeks after the last therapeutic vaccination, mice (n = 4 mice/group) were euthanized, and lungs were harvested for flow cytometric analysis. (A) Percentage of CD4 + CD44+ resident memory T cells (Trm); (B) Absolute number of CD4 + CD44+ resident memory T cells (Trm). Dots represent individual mice, with bars as the mean, and whiskers indicating SD. Asterisks indicate statistical significance (^*p < 0.05, ^**p < 0.01) in mice given therapeutic immunization versus no immunization, using one-way ANOVA with Bonferroni’s multiple comparisons test.