Figure 4.

Multiple stages of differentiated CD4+ T cells in the lungs in mice immunized with ID93 combined with GLA liposomes (either neutral or anionic) combined with QS-21. Four weeks after the last therapeutic vaccination, mice (n = 4 mice/group) were euthanized, and lungs were harvested for flow cytometric analysis. Dots represent individual mice, with bars as the mean, and whiskers indicating SD. Asterisks indicate statistical significance (^*p < 0.05, ^**p < 0.01, ^****p < 0.0001) in mice given therapeutic immunization versus no immunization, using one-way ANOVA with Bonferroni’s multiple comparisons correction. (A) PD1 + KLRG1—(less differentiated, increased proliferation); (B) PD1 + KLRG1+ (short-lived, cytokine-producing, effector T cells); (C) PD1-KLRG1+ (terminally differentiated, cytokine-producing T cells); and (D) PD1-KLRG1−.