FIGURE 4.

Conserved branchpoint sequences (BPS) of minor introns is essential for canonical splicing. (A) LZTR1 exon18–minor intron–exon19 minigene reporter plasmids with indicated mutations were generated. WT, wild‐type. (B) RT‐PCR of LZTR1 minigene with specific primers for the minigene reporter. The mutants in red induced significant intron retention. (C) Examples of genetic variants at BPS and 3′ splice sites (ss) in representative minor intron‐containing genes (MIGs) (PTEN, PARP1, and LZTR1)