FIGURE 1.

Hsa_circ_0129047 was downregulated in LUAD tissues and cell lines. (A) Real‐time quantitative reverse transcription‐polymerase chain reaction (RT‐qPCR) was used to measure hsa_circ_0129047 expression in tissues from 40 LUAD patients and normal lung tissues. (B) hsa_circ_0129047 expression level in LUAD cell lines (A549, Calu‐3, PC9 and H1975) and normal lung cell line (BEAS‐2B) was detected by RT‐qPCR. *p < 0.05, **p < 0.001 vs. BEAS‐2B. (C) Subcellular fractionation analysis was conducted to determine the subcellular localization of hsa_circ_0129047 in LUAD. (D) Hsa_circ_0129047 and circ_0129047 mRNA expression was detected by RT‐qPCR after RNase R treatment. **p < 0.001 vs. Control