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. 2022 Jul 31;36(9):e24591. doi: 10.1002/jcla.24591

FIGURE 4.

FIGURE 4

Hsa_circ_0129047 acted as a sponge for miR‐375. (A) The binding site between hsa_circ_0129047 and miR‐375 was predicted by circInteractome (https://ngdc.cncb.ac.cn/databasecommons/database/id/4778). (B) Luciferase reporter experiment was performed to validate the interaction of miR‐375 and hsa_circ_0129047 in the A549 and Calu‐3 cells. **p < 0.001 vs. miR‐NC. (C) RIP assay was used to validate the interaction between hsa_circ_0129047 and miR‐375. **p < 0.001 vs. anti‐IgG. (D) MiR‐375 expression level in LUAD tissues and normal adjacent tissues were detected by RT‐qPCR. (E) RT‐qPCR was conducted to measure miR‐375 expression level in LUAD cell lines (A549 and Calu‐3) and normal lung cell line (BEAS‐2B). **p < 0.001 vs. BEAS‐2B. (F) Correlation between hsa_circ_0129047 and miR‐375 was determined via Pearson's correlation coefficient. (G) The expression of miR‐375 in A549 and Calu‐3 cells transfected with Oe‐NC, mimic‐NC, Oe‐circ, mimic, and Oe + mimic was detected by RT‐qPCR. *p < 0.05 and **p < 0.001 vs. Oe‐NC; # p < 0.05 and ## p < 0.001 vs. miR‐NC; & p < 0.05 and && p < 0.001 vs. miR‐375 mimic