TABLE 3.
Mass spectrometry condition parameters (ESI+) positive ion mode and mass spectrometry condition parameters (ESI−) negative ion mode
| Scan mode | ||
|---|---|---|
| Full scan (TOF MS) | IDA (Product Ion) | |
| (ESI+) | ||
| Quality range | m/z 601,200 Da | m/z 251,200 Da |
| Atomizing gas (GS1) | 60 psi | 60 psi |
| Auxiliary heating gas (GS2) | 60 psi | 60 psi |
| Curtain air (CUR) | 35 psi | 35 psi |
| Temperature (TEM) | 600°C | 600°C |
| Capillary voltage (IS) | 5000 V | 5000 V |
| Declustering voltage (DP) | 60 V | 60 V |
| Collision energy (CE) | 10 V | 30 V |
| (ESI−) | ||
| Quality range | m/z 601,200 Da | m/z 251,200 Da |
| Atomizing gas (GS1) | 60 psi | 60 psi |
| Auxiliary heating gas (GS2) | 60 psi | 60 psi |
| Curtain air (CUR) | 35 psi | 35 psi |
| Temperature (TEM) | 600°C | 600°C |
| Capillary voltage (IS) | 4000 V | 5000 V |
| Declustering voltage (DP) | 60 V | 60 V |