Fig. 4.

The effect of cathepsin V inhibitors on MCF7 cell proliferation. (A) Cell proliferation monitored as relative carboxyfluorescein succinimidyl ester (CFSE) intensity of CFSE-labeled MCF7 cells in the presence of cathepsin inhibitors compared to DMSO, calculated from data obtained from flow cytometry. (B) CFSE fluorescence intensity after treatment with DMSO (0.1 %, dotted black line, light gray) or E-64d (10 μM), pepstatin (20 μM), LHVS (10 nM), CLIK-148 (1 μM, all solid gray lines), compound 7 (10 μM, blue), or their combinations, as monitored by flow cytometry. Dark gray histograms denote unlabeled cells. (C) The effects of treatments on cell viability as monitored by 7-aminoactinomycin D (7-AAD) staining. The intensity of green CFSE fluorescence was monitored for viable cells only. Data are presented as mean ± SEM (n = 3). ****P < 0.0001 (one-way ANOVA). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)