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. 2022 Sep 2;21:307–321. doi: 10.1016/j.reth.2022.08.006

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© 2022 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 9 — Histological analysis of bone regeneration in vivo (A) Hematoxylin–eosin (HE) staining, Masson staining, immunohistochemistry of Col-1 and CD31 and immunohistofluorescence of Col-1 (B) New bone formation area quantified by Masson staining (n = 6) (C–D) Expression of COL-1 and CD31 quantified by immunohistochemistry (n = 6) (E) Expression of COL-1 quantified by immunohistofluorescence (n = 6). F, fibrous tissue; C, type I collagen; TB, trabecular bone; NB, new bone; Black arrow, remaining scaffold; Black star, transition zone between the remaining scaffold and the new bone. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared with the BM group, #p < 0.05, ##p < 0.01 compared with the BM-SCN group.