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The effect of propranolol on intracellular pH and NHE1 expression in vitro. (A, B) Intracellular pH of MB49 and 5637 was detected by BCECF fluorescent probe with different concentration of propranolol in 24 h. (C) The expression of NHE1 protein in MB49 treated with propranolol (80 µM) was detected by western blot and quantification of NHE1. *, P<0.05. Experiments were repeated three times. NHE1, Na+/H+ exchanger; BCECF, 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein.
