Figure 4.

Implication of GPx1 in WAT oxidative balance. Antioxidant activity of GPx-1 (a). Expression of GPx-1 (expressed as percent arbitrary relative units, referring to values in control animals, which were defined as 100%) (b). Representative Western blot for the expression of GPx-1 and β-actin (as load control) (c). Lipid oxidation expressed by the levels of malondialdehyde (MDA) (d). Protein oxidation expressed by the levels of carbonyl groups (CGs) (e). The results were expressed as means ± SEMs and analyzed using a multifactorial one-way ANOVA followed by Tukey’s test. The number of animals in each group was 6. SEM: standard error of the mean. Groups: C, control group; S, low-selenite group; NS, low-selenite-nanoparticle group; NSS, moderate-selenite-nanoparticle group. Significance: vs. C, * p < 0.05, ** p < 0.01; vs. NP, p p < 0.05.