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. 2022 Aug 31;14(17):3593. doi: 10.3390/nu14173593

Figure 5.

Figure 5

Effects of CoQ10 on the expression of mutant ataxin-3, Hsp27, autophagy and antioxidant protein expression in the cerebellum of 84Q SCA3 mice. (A) At the age of 16 months, the ataxin-3 expression of cerebellar extracts and (B) cerebellar tissue were analyzed by Western blot and immunobiological staining, respectively. Ataxin-3 expression in 50 Purkinje cells of the cerebellar cortex was quantified per group by collecting the magnification images shown in the upper right corner of the figure. (A) The expression of Hsp27 and (C) autophagy marker proteins, including p62, Beclin-1 and LC3-II, and (D) antioxidant proteins, including HO-1, GPx1, GPx2, SOD1 and SOD2 in cerebellar extracts, were analyzed by Western blot. All protein expressions were calculated by normalizing the internal control (GAPDH) prior to a comparison between groups. Values from treated mice were normalized to those of the 84Q SCA3 group. Data are presented as the mean ± SD. * p < 0.05 indicates the WT group compared to the 84Q SCA3 group. # p < 0.05 indicates the 84Q SCA3 + CoQ10 group compared to the 84Q SCA3 group. + p < 0.05 indicates the 84Q SCA3 + CoQ10 group compared to the WT group. WT, wild-type controls; 84Q SCA3, chow diet of vehicle; 84Q SCA3 + CoQ10, CoQ10 supplementation.