Table 4.
Change in viable cell count of probiotics grown on fruits and vegetable matrices.
Non-Dairy Substrate | Probiotic | Change in Cell Density/Viable Cell Count | Reference |
---|---|---|---|
Coconut water | Bacillus coagulans MTCC 5856 spore | After 2 days of fermentation the cell density of Bacillus coagulans at 540 nm increased from 0.121 to 0.683, corresponding to viable cell count of 109 CFU/mL (cell density higher than 0.600). | [30] |
Lactobacillus acidophilus L10 and Lactobacillus casei L26 | Both strains showed increase in viable cell count during the 2 days’ fermentation where L. acidophilus showed higher growth at 3.58 × 108 CFU/mL compared to L. casei at 1.41 × 108 CFU/mL on day 2. | [157] | |
Coconut water + inulin | Lactobacillus plantarum BG 112 | The viable cell count of 9 log CFU/mL of the starting inoculum dropped to a range of 6.00 to 8.70 log CFU/mL depending on the temperature after 16 h of fermentation. Fermentation at temperature 32 °C supplied with 0.5% (w/v) inulin retained the highest viable cells at 8.85 log CFU/mL. | [158] |
Industrialized and fresh coconut water | Lactobacillus casei shirota | After 48 h of fermentation at 36 °C, Lactobacillus casei shirota experienced an increase in viable cell count from 4.15 × 107 CFU/mL to 7.56 × 108 CFU/mL in industrialized coconut water but in fresh coconut water, the cell count increased from 5.4 × 107 CFU/mL (0 h) to 2.5 × 109 CFU/mL (6 h) but no observable cell colony from 18 h to 48 h incubation period. | [159] |
Coconut milk | Lactobacillus acidophilus | The viability of L. acidophilus at initial viable cell count log 4.32 CFU/mL increased after 4 h of fermentation in coconut milk at 37 °C and reached maximum viable cell count of log 9.89 CFU/mL at 20 h and remain constant until 24 h. | [18] |
Breadfruit supernatant | L. acidophilus, L. casei, and L. plantarum DPC 206 | L. acidophilus, L. casei, and L. plantarum DPC 206 showed increased in viable cell count from 5.275 to 8.029 log CFU/mL, 6.055 to 7.952 log CFU/mL, and 5.555 to 7.764 log CFU/mL, respectively, after 72 h of fermentation in breadfruit supernatant at 37 °C. | [69] |
Mango juice and sapota juice | Lactobacillus plantarum NCDC LP 20 | Increased viable cell count from 105 CFU/mL to 8.1 × 108 CFU/mL in mango juice and to 8.0 × 108 CFU/mL in sapota juice after 72 h incubation at 30 °C. | [68] |
Cabbage juice | L. casei A4, L. debrueckii D7 and L. plantarum C3 | L. casei A4, L. debrueckii D7, and L. plantarum C3 showed increase in viable cell count from 3.0 × 106 to 11 × 108 CFU/mL, 4.3 × 105 to 11 × 108 CFU/mL, and 8.0 × 105 to 7.05 × 108 CFU/mL, respectively, after 48 h incubation at 30 °C. | [19] |
Watermelon and tomato juice | L. fermentum and L. casei | Each probiotic shows excellent growth at 37 °C in watermelon and tomato juice combination where L. fermentum and L. casei showed increase in viable cell count from 4.6 × 107 to 2.3 × 108 CFU/mL and 2.7 × 107 to 9.4 × 108 CFU/mL, respectively, after 72 h of fermentation. | [160] |
Pomegranate juice + grape juice + tomato juice + pomegranate peel extract | L. plantarum and L. delbrueckii | When the pomegranate juice is combined with 10% (v/v) grape juice, 5% (v/v) tomato juice, 0.1% (v/v) pomegranate peel extract and added with 2.0 g/L glucose, both strains achieved the highest survival rate with cell count 4.74 × 106 CFU/mL and 4 × 106 CFU/mL of L. plantarum and L. delbrueckii, respectively. | [67] |