Figure 3.

CB1 antagonist DIO intervention promotes CB2-independent weight loss and reduced food intake

Male C57BL/6 WT and CB2^−/− mice were fed 60% HFD for 12 weeks to induce DIO and then treated with 10 mg/kg body weight of AM251 daily for 3 weeks.

(A) Body weight during the 3-week DIO intervention.

(B) DEXA fat mass post 3-week intervention.

(C) Means of daily calorie intake per mouse during the 3-week intervention.

Data are mean ± SEM or box and whisker plots with individual points representing biological replicates. For A-B, N = 5 mice/group except N = 4 mice/group for CB2^−/− HFD + Veh. For C, N = 2 cages of mice. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, by one- or two-way ANOVA with Tukey post-hoc tests.