Figure 4.

CB1 knockout in hematopoietic cells worsens HFD-induced obesity and adipose tissue inflammation

Irradiated B6 CD45.1 (CB1 WT) and CB1^−/− (CB1 KO) male mice were adoptively transferred with donor bone marrow to generate chimeric mice. Reconstituted mice were fed HFD for 16 weeks.

(A) Body weight growth curves during 16 weeks of diet.

(B) DEXA lean mass at 0 and 16 weeks on diet.

(C) DEXA fat mass at 0 and 16 weeks on diet.

(D) Weekly means of daily calorie intake per mouse.

(E) Oral GTT performed during the 16^th week of diet.

(F) Epididymal fat pad wet weight.

(G) Quantification of ATMs per gram of epididymal fat.

(H) Quantification of M1 ATMs per gram of epididymal fat.

(I) Percent of ATMs expressing CD45.1 or CD45.2. Data are mean ± SEM with individual points representing biological replicates. N = 8 mice/chimeric group or 5 mice/syngeneic control group. For D, N = 2 cages. ∗p < 0.05, ∗∗∗∗p < 0.0001 by one-way ANOVA with Tukey post-hoc tests. p < 0.05 if alphabetical characters differ between groups. See also Figures S1–S3.