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. 2022 Aug 24;25(9):104994. doi: 10.1016/j.isci.2022.104994

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Immune CB1 deletion promotes myeloid neuroinflammation

Chimeric mice were generated as described in Figure 4 legend. After 16 weeks of HFD, brain mononuclear cells were analyzed by flow cytometry.

(A) Representative flow cytometry contour plots of CD45⁺CD11b⁺ myeloid immune cell populations. A- CD45⁺Ly-6C⁻ resting microglia, B- CD45^loLy-6C^hi activated microglia, C- CD45^hiLy-6C⁺ infiltrating macrophages.

(B) Resting microglia numbers per brain and percentage of resting microglia expressing CD45.1 or CD45.2 alleles.

(C) Activated microglia numbers per brain and percentage of resting microglia expressing CD45.1 or CD45.2 alleles.

(D) Macrophage numbers per brain and percentage of resting microglia expressing CD45.1 or CD45.2 alleles. Data are mean ± SEM with individual points representing biological replicates. N = 8 mice/chimeric group or 5 mice/syngeneic control group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, by one-way ANOVA with Tukey post-hoc tests. p < 0.05 if alphabetical characters differ between groups.